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Registros recuperados: 7
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Determination of aspartate kinase activity in maize tissues Scientia Agricola
Ferreira,Renato Rodrigues; Vendemiatti,Ariane; Gratão,Priscila Lupino; Lea,Peter John; Azevedo,Ricardo Antunes.
Lysine, threonine, methionine and isoleucine are synthesized from aspartate in a branched pathway in higher plants. Aspartate kinase plays a key role in the control of the aspartate pathway. The enzyme is very sensitive to manipulation and storage and the hydroxamate assay normally used to determine aspartate kinase activity has to be altered according to the plant species and tissue to be analyzed. We have optimized the assay for the determination of aspartate kinase in maize plants callus cell cultures. Among all the assay parameters tested, the concentration of ATP/Mg and temperature were critical for enzyme activity. In the case of temperature, 35°C was shown to be the optimum temperature for aspartate kinase activity.
Tipo: Info:eu-repo/semantics/report Palavras-chave: Aspartate pathway; Maize; Lysine; Threonine; Methionine.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162005000200015
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Diallelic analysis for lysine and oil contents in maize grains Scientia Agricola
Medici,Leonardo Oliveira; Gaziola,Salete Aparecida; Varisi,Vanderlei Aparecido; Paula,José Antonio Carmezini de; Ferreira,Renato Rodrigues; Azevedo,Ricardo Antunes.
Six S5 lines of maize, with differences for lysine and oil contents in grains, were used to carry out a complete series of diallelic crosses. The resulting 15 hybrids were grown in a field at two nitrogen levels (10 and 130 kg N ha-1). The general combining ability (GCA) and specific combining ability (SCA) were obtained by using the method 4, model I of Griffing for grain yield, and grain lysine and oil contents. Significant (p < 0.001) interaction was observed between GCA and N levels for grain yield, indicating the selection of different lines for each N level. This interaction was not significant for lysine content, but there were significant effects of GCA for this trait at both N levels (p < 0.1). Significant effects were not observed for GCA...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cereal; Opaque; Nitrogen; Proteins; Near infrared.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162009000200009
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Expression profile of oxidative and antioxidative stress enzymes based on ESTs approach of citrus Genet. Mol. Biol.
Peroni,Luis Antonio; Ferreira,Renato Rodrigues; Figueira,Antonio; Machado,Marcos Antonio; Stach-Machado,Dagmar Ruth.
Plants not only evolve but also reduce oxygen in photosynthesis. An inevitable consequence of this normal process is the production of reactive oxygen species (ROS). Plants are adequately protected by the presence of multiple antioxidative enzymes in the cytosol and also in the different cell organelles such as chloroplasts, mitochondria, and peroxisomes. Traditionally, ROS were considered to be only a toxic byproduct of aerobic metabolism. However, recently it has become apparent that plants actively produce these molecules which may control many different physiological processes such as abiotic and biotic stress response, pathogen defense and systemic signaling. The search results using the Citrus Genome Program in Brazil (CitEST) for oxidative stress...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Citrus; Genome; EST; Reactive oxygen species; Oxidative stress enzyme.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500016
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Herbicide selectivity by differential metabolism: considerations for reducing crop damages Scientia Agricola
Carvalho,Saul Jorge Pinto de; Nicolai,Marcelo; Ferreira,Renato Rodrigues; Figueira,Antonio Vargas de Oliveira; Christoffoleti,Pedro Jacob.
Herbicide selectivity is an agricultural technology largely exploited in chemical strategies of weed control. The joint action of several protection mechanisms avoids phytotoxicity from herbicide treatment, maintaining the level of agronomically accepted damage to a minimum, or even totally avoiding them. The major mechanism of herbicide selectivity derives from the differential metabolism between weed and crop plant species, with weeds presenting a limited ability to perform it under agronomically recommended conditions. In this case, phytotoxicity can be interpreted as an overcoming of the maximum protection capacity offered by the mechanisms of selectivity, or when considering metabolism as the main factor, the overcoming of the inherent plant ability...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phytotoxicity; Injuries; Yield; Experimental design; Crop management.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162009000100020
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Isolation of enzymes involved in threonine biosynthesis from sorghum seeds Braz. J. Plant Physiol.
Ferreira,Renato Rodrigues; Vendemiatti,Ariane; Meinhardt,Lyndel Wayne; Lea,Peter John; Azevedo,Ricardo Antunes.
Cereal seeds are poor in essential amino acids, particularly lysine, tryptophan and threonine. The amino acids lysine and threonine are synthesized in the aspartate pathway. Although most of the enzymes of the aspartate pathway have been isolated and characterized in higher plant species, the metabolism of lysine and threonine is totally unknown in sorghum. We have isolated two enzymes, aspartate kinase (AK) and homoserine dehydrogenase (HSDH) from sorghum. Optimum assay conditions were established for the determination of AK and HSDH activities. The highest level of activity was observed in immature seeds. AK was shown to be inhibited by threonine and lysine indicating the existence of at least two isoenzymes, one sensitive to threonine inhibition and the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amino acids; Aspartate kinase; Homoserine dehydrogenase; Lysine; Threonine.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202004000200005
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Lysine catabolism: flow, metabolic role and regulation Braz. J. Plant Physiol.
Fornazier,Ricardo Francisco; Azevedo,Ricardo Antunes; Ferreira,Renato Rodrigues; Varisi,Vanderlei Aparecido.
Lysine is an essential amino acid, synthesized in plants in the aspartic acid pathway. The lysine catabolism is performed by the action of two consecutive enzymes, lysine 2-oxoglutarate reductase (LOR) and saccharopine dehydrogenase (SDH). The steady state of lysine is controlled by both, synthesis and catabolism rates, with the final soluble lysine concentration in cereal seeds a direct result of these processes. In the last 40 years, the enzymes involved in lysine biosynthesis have been purified and characterized from some plant species such as carrot, maize, barley, rice, and coix. Recent reports have revealed that lysine degradation might be related to various physiological processes, for instance growth, development and response to environmental...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amino acids; Lysine; Metabolism; Mutants.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202003000100002
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Tolerância diferencial de variedades de cana-de-açúcar a estresse por herbicidas Bragantia
Ferreira,Renato Rodrigues; Oliveira,Fabrício Tadeu Rodrigues de; Delite,Fabrício de Souza; Azevedo,Ricardo Antunes; Nicolai,Marcelo; Carvalho,Saul Jorge Pinto de; Christoffoleti,Pedro Jacob; Figueira,Antonio Vargas De Oliveira.
Este trabalho foi desenvolvido com o objetivo de realizar triagem primária para caracterização da tolerância de variedades SP e CTC de cana-de-açúcar à aplicação de dez herbicidas comumente recomendados para manejo de plantas daninhas nesta cultura. Dois experimentos foram desenvolvidos com esquema fatorial entre variedades de cana-de-açúcar e onze tratamentos herbicidas. No primeiro experimento utilizaram-se sete variedades SP (fatorial 7 x 11): SP80-1842, SP80-3280, SP83-2847, SP87-344, SP87-396, SP89-1115 e SP90-3414. No segundo experimento, foram avaliadas seis variedades CTC (fatorial 6 x 11): CTC1, CTC2, CTC3, CTC4, CTC5 e CTC6. Os tratamentos herbicidas aplicados em pós-emergência inicial foram: ametryn, ametryn + trifloxysulfuron-sodium, clomazone,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Índice SPAD; Herbicidas; Fitotoxicidade; Saccharum.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052010000200019
Registros recuperados: 7
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